Jiini beta-glucosidase (bgl3) lati Streptomyces sp.QM-B814 (Akojọpọ Aṣa Iru Amẹrika 11238) ti jẹ cloned nipasẹ imudara iṣẹ ṣiṣe ti beta-glucosidase-odi mutant ti Streptomyces lividans.Fireemu kika kika ti awọn nucleotides 1440 ti o ṣe koodu polypeptide ti 479 amino acids ni a rii nipasẹ tito lẹsẹsẹ.Amuaradagba ti a fi koodu pamọ (Bgl3) ṣe afihan ibajọra pupọ (ju 45% idanimọ) pẹlu beta-glycosidases lati idile-1 glycosyl hydrolases.Enzymu ti cloned, ti sọ di mimọ ni atẹle ammonium sulphate ojoriro ati awọn igbesẹ chromatographic meji, jẹ monomeric pẹlu ibi-ara molikula 52.6 kDa, gẹgẹbi a ti pinnu nipasẹ spectrometry pupọ, ati aaye isoelectric ti pI 4.4.Enzymu naa han lati jẹ beta-glucosidase pẹlu iyasọtọ sobusitireti gbooro, ti nṣiṣe lọwọ lori awọn celloligomer, o si ṣe awọn aati transglycosylation.Awọn iye km ti o han gbangba ti a pinnu fun p-nitrophenyl-beta-D-glucopyranoside ati cellobiose jẹ 0.27 mM ati 7.9 mM, lẹsẹsẹ.Awọn iye Ki fun glukosi ati delta-gluconolactone, ni lilo p-nitrophenyl-beta-D-glucopyranoside bi sobusitireti, jẹ 65 mM ati 0.08 mM, ni atele.Enzymu ti a sọ di mimọ ni pH ti o dara julọ ti pH 6.5 ati iwọn otutu ti o dara julọ fun iṣẹ ṣiṣe jẹ awọn iwọn 50
