Jiini β-glucosidase aramada kan, bgl1G5, jẹ cloned lati Phalophora sp.G5 ati ni ifijišẹ kosile ni Pichia pastoris.Atupalẹ ọkọọkan tọkasi pe apilẹṣẹ naa ni 1,431-bp fireemu kika ṣiṣii ti n ṣe koodu koodu amuaradagba ti 476 amino acids.Ilana amino acid ti o yọkuro ti bgl1G5 ṣe afihan idanimọ giga ti 85% pẹlu β-glucosidase ti o ni ijuwe lati Humicola grisea ti idile glycoside hydrolase 1. Ti a bawe pẹlu awọn ẹlẹgbẹ olu miiran, Bgl1G5 ṣe afihan iṣẹ ṣiṣe ti o dara julọ ni pH 6.0 ati 50 °C ati pe o jẹ iduroṣinṣin. ni pH 5.0-9.0.Pẹlupẹlu, Bgl1G5 ṣe afihan iwọn otutu ti o dara ni 50 °C (6 wakati idaji-aye) ati iṣẹ ṣiṣe kan pato ti o ga julọ (54.9 U mg-1).Awọn iye K m ati V max si p-nitrophenyl β-D-glucopyranoside (pNPG) jẹ 0.33 mM ati 103.1 μmol min-1 mg-1, lẹsẹsẹ.Ayẹwo iyasọtọ sobusitireti fihan pe Bgl1G5 n ṣiṣẹ gaan lodi si pNPG, alailagbara lori p-nitrophenyl β-D-cellobioside (pNPC) ati p-nitrophenyl-β-D-galactopyranoside (ONPG), ati pe ko ni iṣẹ ṣiṣe lori cellobiose.
