ONPG CAS: 369-07-3 98.0% Min Funfun Lati Paa - Lulú funfun
| Nọmba katalogi | XD90006 |
| CAS | 369-07-3 |
| Orukọ ọja | ONPG(2-Nitrophenyl-beta-D-galactopyranoside) |
| Ilana molikula | C12H15NO8 |
| Òṣuwọn Molikula | 301.25 |
| Awọn alaye ipamọ | 2 si 8 °C |
| Ti irẹpọ Owo idiyele koodu | 29400000 |
Ọja Specification
| Mimọ (HPLC) | Min.98.0% |
| Ifarahan | Funfun lati pa -funfun lulú |
| Ojutu(1% ninu omi) | Ko o, ti ko ni awọ si ojutu ofeefee diẹ |
| Omi akoonu(Karl Fisher) | O pọju.0.5% |
| Yiyi opitika kan pato [α]D20(c=1, H2O) | - 65.0 ° C si -73.0 ° C |
Ifọrọwanilẹnuwo lori idanwo ONPG (idanwo β-galactosidase)
Awọn ibeere ti dide nigbagbogbo laipẹ: 1. Kini idi ti idanwo ONPG le ṣee lo lati ṣe iyatọ bakteria lactose idaduro?2. Kini idi ti boṣewa orilẹ-ede sọ pe o jẹ dandan lati lo 3% sodium chloride trisaccharide iron (tabi iron trisaccharide) fun idanwo ONPG?3. Fun Vibrio parahaemolyticus, nigbati o ba nṣe idanwo OPNG, kilode ti o yẹ ki a fi toluene kun dropwise ni ibamu si boṣewa?Kini iṣẹ naa?
Ile-iṣẹ wa ti ṣe atunyẹwo ọpọlọpọ alaye, ṣe akopọ, o si pin pẹlu rẹ ni isalẹ:
Ilana: Orukọ Kannada ti ONPG jẹ o-nitrobenzene-β-D-galactopyranoside.ONPG le jẹ hydrolyzed sinu galactose ati ofeefee o-nitrophenol (ONP) nipasẹ β-galactosidase, nitorinaa iṣẹ-ṣiṣe ti β-galactosidase ni a le rii nipasẹ iyipada awọ ti alabọde aṣa.
Lactose jẹ suga ti ọpọlọpọ awọn microorganisms nilo lati rii.Awọn iṣelọpọ agbara rẹ nilo awọn enzymu meji, ọkan jẹ permease sẹẹli, lactose wọ inu awọn sẹẹli labẹ iṣẹ ti permease;ekeji ni β-galactosidase, eyiti o ṣe hydrolyzs lactose sinu galactose.Lactose ati glukosi.β-Galactosidase tun le ṣiṣẹ taara lori ONPG lati ṣe hydrolyze o sinu galactose ati ofeefee o-nitrophenol (ONP).O le ṣee ṣe ni wakati 24, paapaa pẹlu awọn fermenters idaduro lactose.Nitorinaa, o ṣe alaye awọn abajade akiyesi ti gbigba aṣa 1 lati agar slant ati inoculating ni kikun Circle ni 36 ° C fun 1-3h ati 24h.Ti a ba ṣe β-galactosidase, yoo tan ofeefee ni 1-3h, ti ko ba si iru enzymu, kii yoo yi awọ pada ni 24h.
Gẹgẹbi awọn enzymu meji ti o wa loke, awọn microorganisms le pin si awọn ẹka wọnyi:
1 lactose-fermenting (wakati 18-24) kokoro arun pẹlu permease ati β-galactosidase P + G +;
2 Awọn fermenters lactose ti o da duro (n gba to ju wakati 24 lọ) aini permease ṣugbọn ti o ni galactosidase: P-G+.
3 Awọn fermenters ti kii ṣe lactose ti ko ni permease mejeeji ati galactosidase: P- G-.
Idanwo ONPG le ṣee lo lati ṣe iyatọ si awọn kokoro arun lactose-lag-fermenting (P-G+) lati awọn kokoro arun lactose ti kii-fermenting (PG-), gẹgẹbi:
1 Ṣe iyatọ awọn fermenters lactose pẹ (P-G+) lati awọn fermenters ti kii ṣe lactose (P- G-).
(a) Citrobacter (+) ati Salmonella arizonae (+) lati Salmonella (-).
(b) Escherichia coli (+) lati Shigella sonnei (-).
Kini idi ti idanwo ONPG ṣe ni lilo aṣa alẹ kan lori 3% sodium kiloraidi ferric trisaccharide (irin trisaccharide)?Ile-iṣẹ wa ti ṣagbero ọpọlọpọ alaye, ṣugbọn ko si alaye asọye.Nikan lori oju opo wẹẹbu FDA, o ti kọwe pe “Awọn aṣa inoculate lati ṣe idanwo lori awọn agar iron agar mẹta suga ati incubate fun 18 h ni 37 ° C (tabi iwọn otutu miiran ti o yẹ, ti o ba nilo) Nutrient (tabi awọn miiran) agar slants ti o ni 1.0 % lactose tun le ṣee lo."tumo si: awọn kokoro arun ti a ṣe inoculated lori trisaccharide iron alabọde ati ki o gbin ni 37 ° C fun 18h.Ounjẹ agar slants (tabi miiran) alabọde ti o ni 1% lactose tun jẹ itẹwọgba.Nitorinaa, a sọ pe alabọde iron trisaccharide ni lactose.Lẹhin idagba oru, awọn kokoro arun ti ṣe agbejade β-galactosidase ti nṣiṣe lọwọ to dara.Lilo iru awọn kokoro arun, ONPG le jẹ ibajẹ nipasẹ β-galactosidase yiyara.Iyalẹnu adanwo ni iyara ati ṣafihan dara julọ.Ni afikun, fifi silẹ wiwu ti toluene ati iwẹ omi fun awọn iṣẹju 5 ni gbogbo rẹ lati le tu β-galactosidase silẹ ni kikun.
