2'-Deoxyuridine Cas: 951-78-0
| Nọmba katalogi | XD90583 |
| Orukọ ọja | 2'-Deoxyuridine |
| CAS | 951-78-0 |
| Ilana molikula | C9H12N2O5 |
| Òṣuwọn Molikula | 228.20 |
| Awọn alaye ipamọ | 2 si 8 °C |
| Ti irẹpọ Owo idiyele koodu | 29349990 |
Ọja Specification
| Ifarahan | Funfun si pa-funfun lulú |
| Ayẹwo | 99% |
| Ojuami Iyo | 164 - 168 Deg C |
| Isonu lori Gbigbe | <1.0% |
| Aloku lori Iginisonu | <0.1% |
Ilọsiwaju lilo nitrogen ninu ẹran jẹ pataki lati le ni aabo iṣelọpọ ẹran alagbero.Gẹgẹbi awọn purines ati pyrimidines (PP) jẹ apakan ti o ni itẹlọrun ti rumen nitrogen, oye ilọsiwaju ti gbigba ati iṣelọpọ agbedemeji ti PP jẹ pataki.Iṣẹ ti o wa lọwọlọwọ ṣe apejuwe idagbasoke ati afọwọsi ti ọna ifura ati pato fun ipinnu igbakana ti 20 purines (adenine, guanine, guanosine, inosine, 2'-deoxyguanosine, 2'-deoxyinosine, xanthine, hypoxanthine), pyrimidines (cytosine, thymine, uracil, cytidine, uridine, thymidine, 2'-deoxyuridine), ati awọn ọja ibajẹ wọn (uric acid, allantoin, β-alanine, β-ureidopropionic acid, β-aminoisobutyric acid) ninu pilasima ẹjẹ ti awọn malu ifunwara.Imọ-ẹrọ orisun-kiromatogirafi iṣẹ ṣiṣe giga pọ si electrospray ionization tandem mass spectrometry (LC-MS/MS) ni idapo pẹlu awọn ajohunše isọdi-ibamu matrix kọọkan ati awọn akopọ itọkasi isotopically aami iduro.T itupale pipo ni iṣaaju nipasẹ ilana itọju-iṣaaju aramada ti o wa pẹlu ojoriro ethanol, sisẹ, evaporation ati atunṣe.Awọn paramita fun Iyapa ati wiwa lakoko itupalẹ LC-MS/MS ni a ṣe iwadii.O ti jẹri pe lilo awoṣe isọdi-iwọle dipo awoṣe isọdi laini kan yorisi CV% kekere ati aini idanwo ibamu ṣe afihan ipadasẹhin laini itẹlọrun.Ọna naa bo awọn sakani ifọkansi fun metabolite kọọkan ni ibamu si iyẹn ninu awọn ayẹwo gangan, fun apẹẹrẹ guanine: 0.10-5.0 μmol/L, ati allantoin: 120-500 μmol/L.CV% fun awọn sakani titobi ti a yan wa labẹ 25%.Ọna naa ni atunṣe to dara (CV% ≤25%) ati iṣedede agbedemeji (CV%≤25%) ati awọn imularada to dara julọ (91-107%).Gbogbo awọn metabolites ṣe afihan iduroṣinṣin igba pipẹ to dara ati iduroṣinṣin to dara laarin awọn ṣiṣe (CV%≤10%).Awọn iwọn oriṣiriṣi ti awọn ipa matrix pipe ni a ṣe akiyesi ni pilasima, ito ati wara.Ipinnu ti awọn ipa matrix ojulumo ṣafihan pe ọna naa dara fun gbogbo awọn metabolites PP ti a ṣe ayẹwo ni pilasima ti a fa lati inu iṣọn-ẹjẹ ati awọn iṣọn ẹdọforo, ẹdọ ẹdọ ati awọn iṣọn gastrosplenic ati, pẹlu awọn imukuro diẹ, tun fun awọn eya miiran bii adie, ẹlẹdẹ, mink, eda eniyan ati eku.
