dCMP, 2′-Deoxycytidine 5′-monophosphate, ọ̀fẹ́ acid CAS:1032-65-1
| Nọmba katalogi | XD90549 |
| Orukọ ọja | dCMP, 2'-Deoxycytidine 5'-monophosphate, acid ofe |
| CAS | 1032-65-1 |
| Ilana molikula | C9H14N3O7P |
| Òṣuwọn Molikula | 307.20 |
| Awọn alaye ipamọ | -15 si -20 °C |
| Ti irẹpọ Owo idiyele koodu | 29349990 |
Ọja Specification
| Ifarahan | ri to |
| Ayẹwo | 99% |
Beta-D-2'-deoxy-2'-fluoro-2'-C-methylcytidine (PSI-6130) jẹ oludena kan pato ti o lagbara ti kokoro jedojedo C (HCV) RNA kolaginni ninu awọn sẹẹli Huh-7.Lati ṣe idiwọ HCV NS5B RNA polymerase, PSI-6130 gbọdọ jẹ phosphorylated si fọọmu 5'-triphosphate.Fọsifọrilation ti PSI-6130 ati idinamọ ti HCV NS5B ni a ṣe iwadii.phosphorylation ti PSI-6130 nipasẹ eda eniyan recombinant 2'-deoxycytidine kinase (dCK) ati uridine-cytidine kinase 1 (UCK-1) ni a ṣe iwọn nipasẹ lilo ifasilẹ spectrophotometric pọ.PSI-6130 ni a fihan lati jẹ sobusitireti fun dCK mimọ, pẹlu km ti 81 microM ati kcat kan ti 0.007 s-1, ṣugbọn kii ṣe sobusitireti fun UCK-1.PSI-6130 monophosphate (PSI-6130-MP) jẹ phosphorylated daradara si difosifeti ati lẹhinna si triphosphate nipasẹ eniyan recombinant UMP-CMP kinase ati nucleoside diphosphate kinase, lẹsẹsẹ.Idinamọ ti iru-igi ati iyipada (S282T) HCV NS5B RNA polymerases ni a ṣe iwadi.Iduroṣinṣin idinamọ igbagbogbo (Ki) fun PSI-61 30 triphosphate (PSI-6130-TP) pẹlu enzymu iru-ẹgan jẹ 4.3 microM.Awọn abajade kanna ni a gba pẹlu 2'-C-methyladenosine triphosphate (Ki = 1.5 microM) ati 2'-C-methylcytidine triphosphate (Ki = 1.6 microM).NS5B pẹlu iyipada S282T, eyiti a mọ lati funni ni resistance si 2'-C-methyladenosine, ni idiwọ nipasẹ PSI-6130-TP bi daradara bi iru egan.Ijọpọ PSI-6130-MP sinu RNA catalyzed nipasẹ mimọ NS5B RNA polymerase yorisi ni ifopinsi pq.
