Awọn ọja

Ninu ọna enzymu iyipada-kinetic angiotensin (ACE), ilowo ati ifipamọ aipe jẹ 80 mmol/L borate buffer ni pH 8.2 (37 iwọn C).Ipele aisun ni a rii ninu iṣesi, ati idawọle iṣẹju 5 kan ti sobusitireti ati pilasima ni a daba ṣaaju wiwọn kainetik.Sobusitireti, N-[3- (2-furyl) acryloyl] -L-phenylalanylglycylglycine (FAPGG), ifọkansi ti wa ni iwọn ni 1.0 mmol/L ati wiwọn wefulenti jẹ ni 345 nm lati rii daju laini wiwọn.Ilana ti a dabaa nlo ipin iwọn didun 1: 9 pilasima-si-reagent.Iwọn ila ila ti idanwo naa gbooro si isunmọ 170 U/L, ti o nsoju 25% hydrolysis sobusitireti.Gbigba FAPGG jẹ ipinnu nipasẹ wiwọn iyatọ ninu gbigba laarin 1.0 mmol/L FAPGG ati awọn solusan ọja.A ṣe ayẹwo iṣotitọ gigun gigun nipasẹ akiyesi iye ifasilẹ ti a nireti ti ojutu FAPGG, ati iyapa 1.0-nm lati 345 nm ṣe iyipada gbigba nipasẹ 15.5%.Ipese awọn idanwo ACE ni isunmọ 60 ati 100 U/L jẹ 3.5% ati 2.4% laarin ipele ati 2.9% ati 2.6% laarin ipele, lẹsẹsẹ.Aarin itọkasi (2.5th si 97.5th percentiles) jẹ 41-139 U/L, ati pe ko si iyatọ laarin awọn iye fun awọn ọkunrin ati awọn obinrin.