γ-L-Glutamyl-3-carboxy-4-nitroanilide, iyọ monoammonium Cas: 63699-78-5 Ina ofeefee microcrystalline lulú
| Nọmba katalogi | XD90183 |
| Orukọ ọja | γ-L-Glutamyl-3-carboxy-4-nitroanilide, iyo monoammonium |
| CAS | 63699-78-5 |
| Ilana molikula | C12H12N3O7-.H4N + |
| Òṣuwọn Molikula | 328.27 |
| Awọn alaye ipamọ | Ibaramu |
Ọja Specification
| Ifarahan | Ina ofeefee microcrystalline lulú |
| Asay | 99% |
| Ojuami yo | 187ºC ± 2.0ºC |
| Oju omi farabale | 718.3°C ni 760 mmHg |
| oju filaṣi | 368,5 °C |
| Solubility | H2O: 100 mg/mL, ko o, ofeefee-alawọ ewe |
Awọn kinetics ti omi ara gamma-glutamyltransferase (EC 2.3.2.2) ni a ṣe iwadii, pẹlu lilo glycylglycine gẹgẹbi sobusitireti itẹwọgba gamma-glutamyl ati gamma-glutamyl-4-nitroanilide ati itọsẹ carboxy rẹ, gamma-glutamyl-3-carboxy-4 -nitroanilide, bi olugbeowosile sobsitireti.Iṣẹlẹ nigbakanna ti gamma-glutamyltransfer mejeeji ati gbigbe ara ẹni jẹ idasilẹ nipasẹ kiromatografi iwe ti o sọkalẹ.Ibakan-ipin awọn igbero ilọpo meji-pada jẹri pe ẹrọ enzymu jẹ eyiti ko ṣe pataki (ping-pong bi-bi).Idinamọ nipasẹ boya oluranlọwọ ko rii, ati idinamọ nipasẹ glycylglycine nikan ni a ṣe akiyesi ni awọn ifọkansi ju awọn anfani ile-iwosan lọ.Awọn iduro kinetic ti a gba nipasẹ itupalẹ isọdọtun aiṣedeede ti data iyara ni ibẹrẹ ni a lo lati pinnu awọn ifọkansi sobusitireti reagent fun idanwo ti henensiamu yii.Ayẹwo pẹlu lilo 4 mmol ti gamma-glutamyl-3-carboxy-4-nitroanilide ati 100 mmol ti glycylglycine fun lita kan pese awọn iṣẹ deede fun awọn ti a ṣe ayẹwo pẹlu lilo 4 mmol ti gamma-glutamyl-4-nitroanilide ati 40 mmol ti glycylglycine fun lita.Awọn ifọkansi wọnyi ti oluranlọwọ carboxy ati glycylglycine tun jẹ “idiwọn ti o dara julọ” ati pe ko ṣafihan awọn iṣoro ilana nigba lilo.
