iroyin

Ọpọlọpọ awọn akikanju ti a ko kọ ni ayika wa, ti o dabi ẹnipe o jẹ lasan, ṣugbọn ni otitọ wọn dakẹ ti ṣe alabapin pupọ si wa.Proteinase K jẹ “akọni ti a ko kọ” ni ile-iṣẹ iwadii molikula, botilẹjẹpe akawe pẹlu “nla ati alagbara” ninu ile-iṣẹ naa, proteinase K jẹ bọtini kekere ti a ti foju foju foju wo pataki rẹ.Pẹlu ibesile ti ajakale ade tuntun, ibeere fun proteinase K ti pọ si, ati pe ipese ni ile ati ni ilu okeere ti jinna lẹhin lilo, ati pe gbogbo eniyan lojiji rii pe proteinase K jẹ pataki.
Kini lilo proteinase K
Proteinase K jẹ protease serine pẹlu iṣẹ-ṣiṣe enzymu proteolytic ati pe o le ṣetọju iṣẹ-ṣiṣe ni ọpọlọpọ awọn agbegbe (pH (4-12.5), fifẹ iyọ-giga, iwọn otutu giga ti 70 ° C, bbl).Ni afikun, iṣẹ-ṣiṣe ti proteinase K ko ni idinamọ nipasẹ SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, bbl, ati iye kan ti detergent tun le mu iṣẹ-ṣiṣe ti proteinase K. Ni itọju egbogi (kokoro ati disinfection microbial). ), ounjẹ (ẹran ẹran), alawọ (irun irun), mimu ọti-waini (ọti-ọti-ọti), igbaradi amino acid (awọn iyẹ ẹyẹ ti o bajẹ), isediwon acid nucleic, ni ipo arabara, ati bẹbẹ lọ, proteinase K Awọn ohun elo wa.Ohun elo ti o wọpọ julọ ni isediwon acid nucleic.
Proteinase K le ṣe enzymolyze gbogbo iru awọn ọlọjẹ ti o wa ninu apẹẹrẹ, pẹlu awọn itan-akọọlẹ wọnyẹn ti o ni asopọ ni wiwọ si awọn acids nucleic, ki awọn acids nucleic le tu silẹ lati inu apẹẹrẹ ati tu silẹ sinu jade, ni irọrun igbesẹ atẹle ti isediwon ati isọdi.Ni wiwa ti gbogun ti nucleic acid, proteinase K jẹ ọkan ninu awọn paati pataki ninu ojutu iṣapẹẹrẹ ọlọjẹ.Proteinase K le kiraki ati aiṣiṣẹ amuaradagba ẹwu ti ọlọjẹ, eyiti o jẹ ailewu lakoko gbigbe ati ipele wiwa;ni afikun, proteinase K tun le degrade RNase idilọwọ awọn ibaje ti gbogun ti RNA ati ki o dẹrọ nucleic acid erin.
Okiki alẹ ti proteinase K
Boya ni aaye ti iwadii ijinle sayensi tabi ni aaye IVD, isediwon acid nucleic jẹ idanwo ipilẹ julọ, nitorinaa proteinase K nigbagbogbo jẹ aye pataki pupọ.Sibẹsibẹ, ni igba atijọ, proteinase K jẹ eyiti a ko mọ daradara ju ipa rẹ lọ.Apa nla ti eyi jẹ nitori ipese ati ibatan ibeere ti proteinase K jẹ iduroṣinṣin pupọ.Diẹ eniyan yoo ro pe ipese proteinase K yoo jẹ iṣoro kan.
Pẹlu ibesile ti ajakale ade tuntun, ibeere fun idanwo acid nucleic ti pọ si.Ni ipari Oṣu Karun ọdun 2020, Ilu China ti pari awọn idanwo ade tuntun 90 miliọnu, ati pe nọmba yii paapaa jẹ itaniji diẹ sii ni iwọn agbaye.Ninu awọn adanwo isediwon acid nucleic, ifọkansi iṣiṣẹ ti proteinase K jẹ nipa 50-200 μg/mL.Ni gbogbogbo, o gba to 100 μg ti proteinase K lati yọ ayẹwo ti acid nucleic jade.Ni lilo gangan, lati le mu iṣẹ ṣiṣe ti isediwon acid nucleic pọ si, nigbagbogbo Proteinase K yoo ṣee lo ni iye ti o pọ si.Wiwa Nucleic acid ti coronavirus tuntun ti mu iye nla ti ibeere proteinase K.Ipese atilẹba ati iwọntunwọnsi eletan ti proteinase K ti bajẹ ni iyara, ati proteinase K di ohun elo idena ajakale-arun pataki ni alẹ kan.
Awọn iṣoro ni iṣelọpọ proteinase K
Botilẹjẹpe pẹlu idagbasoke ti ajakale-arun, iye pataki ti proteinase K ti ni idiyele nipasẹ awọn eniyan, o jẹ itiju pe nitori bọtini kekere ti proteinase K, diẹ ninu awọn ile-iṣẹ ile ti kopa ninu iṣelọpọ proteinase K. Nigbati awọn eniyan fẹ lati fi idi proteinase K iṣelọpọ Lakoko ilana iṣelọpọ, o ṣe awari pe proteinase K jẹ amuaradagba pataki pupọ.O jẹ nija pupọ lati faagun agbara iṣelọpọ ti proteinase K ni igba diẹ.
Iṣelọpọ iwọn-nla ti proteinase K dojukọ awọn iṣoro wọnyi
1. Low ikosile
Amuaradagba K le ti kii-ni pato ba awọn ọlọjẹ pupọ julọ jẹ ki o fa majele to ṣe pataki si sẹẹli ogun ikosile.Nitorinaa, ipele ikosile ti proteinase K jẹ kekere pupọ.Ṣiṣayẹwo awọn eto ikosile ati awọn igara ti o ṣafihan proteinase K ni gbogbogbo nilo gigun gigun.
2. Awọn iṣẹku ti pigments ati nucleic acids
Bakteria-tobi ṣafihan iye nla ti pigmenti ati awọn iṣẹku acid nucleic ogun.O ti wa ni soro lati yọ awọn wọnyi impurities pẹlu kan awọn ìwẹnu ilana, ati eka ìwẹnumọ mu ki awọn iye owo ati ki o din imularada oṣuwọn.
3. Aisedeede
Proteinase K ko ni iduroṣinṣin to, o le enzymolyze funrararẹ, ati pe o nira lati tọju rẹ ni iduroṣinṣin ni 37 ° C fun igba pipẹ laisi oluranlowo aabo.
4. Rọrun lati ṣaju
Nigbati o ba ngbaradi awọn didi-si dahùn o lulú ti proteinase K, ni ibere lati rii daju wipe awọn ri to akoonu ti proteinase K ninu awọn di-si dahùn o lulú jẹ tobi, o jẹ pataki lati fi kan di-si dahùn o aabo oluranlowo ni kan to ga fojusi, ṣugbọn nigbati awọn ifọkansi ti proteinase K de 20mg / milimita ati loke, o rọrun Akopọ n ṣe itọsi, eyiti o mu awọn iṣoro nla wa si didi-gbigbe ti proteinase K pẹlu akoonu to lagbara.
5. Idoko-owo nla
Proteinase K ni iṣẹ-ṣiṣe protease ti o lagbara ati pe o le ṣe hydrolyze awọn protease miiran ninu yàrá.Nitorinaa, proteinase K nilo awọn agbegbe iṣelọpọ amọja, ohun elo, ati oṣiṣẹ fun iwadii ati idagbasoke ati iṣelọpọ.
XD BIOCHEM's proteinase K ojutu
XD BIOCHEM has a mature protein expression and purification platform, and has rich experience in the expression and purification of recombinant proteins and optimization of production processes. Through the rapid formation of a research and development team, the large-scale production process of proteinase K has been overcome. The monthly output of freeze-dried powder is more than 30 KG. The product has stable performance, high enzyme specific activity, and no host cytochrome and nucleic acid residues. Welcome to contact XD BIOCHEM Obtain a trial package (E-mail: sales@xdbiochem.com Tel: +86 513 81163739).
Awọn solusan imọ-ẹrọ XD BIOCHEM pẹlu
Lilo iṣọpọ plasmid pupọ-daakọ, awọn igara ikosile ti o ga pẹlu ipele ikosile ti 8g / L ti yan, eyiti o bori iṣoro ti ipele ikosile kekere ti proteinase K;
Nipasẹ idasile ilana isọdi-ọpọ-igbesẹ, cytochrome ogun ati awọn iṣẹku acid nucleic ti proteinase K ti yọkuro ni aṣeyọri ni isalẹ iye iwọn;
Nipasẹ ibojuwo-giga ti awọn agbekalẹ ifipamọ aabo, ifipamọ ti o le tọju proteinase K ni iduroṣinṣin ni 37 ° C ti yan;
Awọn buffers iboju bori iṣoro naa pe proteinase K rọrun lati ṣajọpọ ati ṣaju ni awọn ifọkansi giga, ati pe o fi ipilẹ le proteinase K akoonu ti o lagbara ti didi-gbigbe.

XD BIOCHEM proteinase K ayẹwo

XD BIOCHEM proteinase K idanwo iduroṣinṣin: kii yoo ni iyipada pataki ninu iṣẹ lẹhin 80 d ni iwọn otutu yara

XD BIOCHEM proteinase K idanwo iduroṣinṣin: kii yoo ni iyipada pataki ninu iṣẹ lẹhin 80 d ni iwọn otutu yara

Ifiwera ipa isediwon acid nucleic ti XD BIOCHEM proteinase K ati awọn ọja idije.Ninu ilana isediwon acid nucleic, XD BIOCHEM ati ifigagbaga proteinase K ni a lo ni atele.Imudara isediwon ti XD BIOCHEM proteinase K ti ga julọ ati pe iye Ct ti jiini ibi-afẹde ti dinku.