L-Proline Cas: 147-85-3 99% funfun lulú
| Nọmba katalogi | XD90293 |
| Orukọ ọja | L-Proline |
| CAS | 147-85-3 |
| Ilana molikula | C5H9NO2 |
| Òṣuwọn Molikula | 115.13046 |
| Awọn alaye ipamọ | Ibaramu |
| Ti irẹpọ Owo idiyele koodu | 29339980 |
Ọja Specification
| Ayẹwo | 99% iṣẹju |
| Ifarahan | funfun lulú |
| Yiyi pato | -84,5 to -86 |
| Awọn irin ti o wuwo | <15ppm |
| AS | <1ppm |
| Ph | 5.9 - 6.9 |
| SO4 | <0.050% |
| Fe | <30ppm |
| Isonu lori Gbigbe | <0.3% |
| Aloku lori Iginisonu | <0.10% |
| NH4 | <0.02% |
| Cl | <0.050% |
| Ipinle ti Solusan | > 98% |
Loye iṣelọpọ ti ogun microbial jẹ pataki fun idagbasoke ati iṣapeye ti gbogbo-cell ti o da lori awọn ilana biocatalytic, bi o ṣe n ṣalaye ṣiṣe iṣelọpọ.Eyi jẹ otitọ paapaa fun biocatalysis redox nibiti awọn sẹẹli ti nṣiṣe lọwọ iṣelọpọ ti wa ni iṣẹ nitori agbara isọdọtun cofactor/cosubstrate endogenous ninu agbalejo.Recombinant Escherichia coli ti a lo fun overproducing proline-4-hydroxylase (P4H), a dioxygenase catalyzing awọn hydroxylation ti free L-proline sinu trans-4-hydroxy-L-proline pẹlu a-ketoglutarate (a-KG) bi cosubstrate.Ninu gbogbo-ẹyin biocatalyst yii, iṣelọpọ erogba aarin n pese cosubstrate a-KG ti o nilo, iṣẹ ṣiṣe biocatalytic P4H taara si iṣelọpọ erogba ati iṣẹ iṣelọpọ.Nipa lilo mejeeji esiperimenta ati awọn irinṣẹ isedale iṣiro, gẹgẹbi imọ-ẹrọ ti iṣelọpọ ati (13) itupalẹ ṣiṣan ti iṣelọpọ ti C-metabolic ((13) C-MFA), a ṣe iwadii ati ni iwọn ti o ṣapejuwe eto-ara, ti iṣelọpọ, ati idahun bioenergetic ti gbogbo sẹẹli biocatalyst. si iyipada bioconversion ti o ni ifọkansi ati idanimọ ti o ṣee ṣe awọn igo igo ti iṣelọpọ fun imọ-ọna ọna onipin siwaju sii.Ibajẹ proline-aipe E. coli igara ni a ṣe nipasẹ piparẹ jiini putA fifi koodu proline dehydrogenase kuro.Gbogbo sẹẹli biotransformations pẹlu igara mutant yii yorisi kii ṣe si pipo proline hydroxylation nikan ṣugbọn tun si ilọpo meji ti oṣuwọn idasile trans-4-L-hydroxyproline (hyp) kan pato, ni akawe si iru egan.Onínọmbà ti ṣiṣan erogba nipasẹ iṣelọpọ aarin ti igara mutant ṣe afihan pe ibeere a-KG ti o pọ si fun iṣẹ ṣiṣe P4H ko mu ṣiṣan a-KG pọ si, ti n ṣe afihan iṣẹ ṣiṣe ọmọ TCA ti o ni wiwọ labẹ awọn ipo ti a ṣe iwadi.Ninu igara iru egan, iṣelọpọ P4H ati catalysis fa idinku ninu ikore baomasi.O yanilenu, igara ΔputA tun san isanpada ATP ti o somọ ati pipadanu NADH nipa idinku awọn ibeere agbara itọju ni awọn iwọn gbigba glukosi kekere ni afiwe, dipo jijẹ iṣẹ TCA. The putA knockout in recombinant E. coli BL21(DE3)(pLysS) jẹ ileri fun iṣelọpọ P4H catalysis kii ṣe ni awọn ofin ti ikore biotransformation nikan, ṣugbọn tun nipa awọn oṣuwọn fun biotransformation ati gbigba proline ati ikore ti hyp lori orisun agbara.Awọn abajade fihan pe, lori knockout putA kan, idapọ ti TCA-cycle si proline hydroxylation nipasẹ cosubstrate a-KG di idinamọ ifosiwewe bọtini ati ibi-afẹde lati mu ilọsiwaju siwaju sii ti a-KG-ti o gbẹkẹle biotransformations.
