FLUORESCEIN MONO-BETA-D-GALACTOPYRANOSIDE Cas: 102286-67-9 99% Iyẹfun funfun
| Nọmba katalogi | XD90047 |
| Orukọ ọja | FLUORESCEIN MONO-BETA-D-GALACTOPYRANOSIDE |
| CAS | 102286-67-9 |
| Ilana molikula | C26H22O10 |
| Òṣuwọn Molikula | 494.12 |
| Awọn alaye ipamọ | Ibaramu |
Ọja Specification
| Ifarahan | funfun lulú |
| Ayẹwo | 99% |
| iwuwo | 1.69g/cm3 |
| SisePororo | 813.4ºC ni 760mmHg |
| FilasiPororo | 281ºC |
| RefractiveIndex | 1.772 |
| Ibi ipamọCawọn ipo | -20ºC |
| OruPifọkanbalẹ | 5.73E-28mmHg ni 25°C |
Monogalactopyranosides ti fluorescein ati fluorescein methyl ester: kolaginni, enzymatic hydrolysis nipasẹ biotnylated β-galactosidase, ati ipinnu ti atupatupọ pinpin kaakiri
Fluorescein monoglycosides (d-galactopyranoside (FMG) ati d-glucopyranoside) ati methyl ester wọn (MFMG) ti pese sile lati acetobromoglucose / galactose ati fluorescein methyl ester ni awọn eso to dara.Awọn adanwo hydrolysis enzymatic (lilo biotinylated β-galactosidase) ti awọn itọsẹ galacto ti ṣe ati pe a ti ṣe iṣiro awọn aye kainetik.Awọn akoko 15-20 ti o pọ si ti fifẹ fluorescence ti ṣe akiyesi lakoko hydrolysis.Alekun laini ti fluorescence ti ṣe akiyesi ni akoko kukuru ati ifọkansi kekere ti sobusitireti, ṣiṣe awọn agbo ogun wọnyi wulo ati awọn iwadii ifura fun galactosidases.Titobi iye Michaelis–Menten ibakan (Km) fun MFMG ga ju ti FMG ni iyanju iyipada imudara ti o ṣeeṣe ti sobusitireti fluorogenic.Iye km fun β-Gal biotinylated pẹlu FMG kere ju iyẹn lọ fun enzymu abinibi.Akiyesi yii tọkasi isunmọ sobusitireti ti o ga julọ ti henensiamu biotinylated ni lafiwe si henensiamu abinibi.Awọn alafojusi itusilẹ itumọ ti ni iwọn, fun awọn sobusitireti fluorogenic mejeeji ati awọn ọja mejeeji, ni lilo sipekitikọpọ ibamu fluorescence.Awọn idaparọ itọka itumọ fun awọn sobusitireti fluorogenic ati awọn ọja hydrolysis enzymatic ti ni iwọn lati jẹ iru, ni iwọn 3.5-4.5 × 10-10 m2 s-1.Nitorinaa imudara tabi idaduro ti awọn kinetikisi enzymatic nitori iyatọ ninu arinbo itumọ ti sobusitireti ati ọja ko han gbangba.
