dCTP, 2′-Deoxycytidine-5′-triphosphoric acid disodium iyọ Cas:102783-51-7 Funfun Powder
| Nọmba katalogi | XD90584 |
| Orukọ ọja | dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium iyọ |
| CAS | 951-78-0 |
| Ilana molikula | C9H12N2O5 |
| Òṣuwọn Molikula | 228.20 |
| Awọn alaye ipamọ | -20 °C |
| Ti irẹpọ Owo idiyele koodu | 29349990 |
Ọja Specification
| Ifarahan | Funfun Powder |
| Ayẹwo | 99% |
Awọn sẹẹli Eukaryotic ni iwọntunwọnsi elege ti awọn oye iṣẹju ti awọn triphosphates deoxyribonucleoside mẹrin (dNTPs), to nikan fun iṣẹju diẹ ti ẹda DNA.Mejeeji aipe ati iyọkuro ti dNTP kan le ja si awọn iwọn iyipada ti o pọ si, atunṣe DNA ti ko tọ tabi idinku DNA mitochondrial.Awọn dNTP ni a maa n ṣe iwọn nipasẹ idanwo enzymatic ninu eyiti iṣakojọpọ dATP ipanilara (tabi dTTP ipanilara ninu idanwo fun dATP) sinu awọn oligonucleotides sintetiki kan pato nipasẹ DNA polymerase jẹ ibamu si ifọkansi ti dNTP aimọ.A rii pe Klenow DNA polymerase ti a lo nigbagbogbo le paarọ ribonucleotide ti o baamu fun dNTP aimọ ti o yorisi ni awọn igba miiran si iwọn apọju nla ti awọn dNTPs.Bayi a ṣe apejuwe awọn ipo idanwo fun dNTP kọọkan ti o yago fun isọdọkan ribonucleotide.Fun awọn idanwo dTTP ati dATP o to lati dinku awọn ifọkansi ti enzymu Klenow ati ti aami dATP (tabi dTTP);fun dCTP ati dGTP a ni lati rọpo enzymu Klenow pẹlu boya Taq DNA polymerase tabi Thermo Sequenase.A daba pe ninu diẹ ninu awọn ijabọ iṣaaju iṣakojọpọ ribonucleotide le ti fa awọn iye ti o ga ju fun dGTP ati dCTP.
