D-Luciferin Cas: 2591-17-5 99% Aisi-funfun si erupẹ ofeefee nbsp BEETLE LUCIFERIN
Nọmba katalogi | XD90248 |
Orukọ ọja | D-Luciferin |
CAS | 2591-17-5 |
Ilana molikula | C11H8N2O3S2 |
Òṣuwọn Molikula | 280.323 |
Awọn alaye ipamọ | -15 si -20 °C |
Ti irẹpọ Owo idiyele koodu | 29342080 |
Ọja Specification
Omi akoonu | O pọju.2.0% |
Turbidity | Iye ti o ga julọ ti 2.0NTU |
Yiyi opitika pato | -36 si -32 |
Ifarahan | Pa-funfun to ofeefee lulú |
HPLC ti nw | Min 99% |
Molar iparun olùsọdipúpọ | min 17900 L/(mol cm) |
Ifihan: D-luciferin jẹ sobusitireti fun adenosine triphosphate (ATP-igbẹkẹle iṣesi bioluminescence.Ilana ti bioluminescence ni pe luciferin jẹ oxidized nipasẹ luciferase ni iwaju ATP ati atẹgun.Ilana ifaseyin kemikali jẹ bi atẹle: ATP+D-Luciferin+O2→Oxyluciferin+AMP+PPi+O2+Light.
Ilana ti iṣe: Ilana ti igbese ti D-luciferin ni pe labẹ iṣẹ ti ATP ati luciferase, luciferin (sobusitireti) le jẹ oxidized lati tan ina.Nigbati luciferin ba pọ ju, nọmba awọn photon ti a ṣe ni ibamu pẹlu ifọkansi ti luciferase.
Ohun elo: D-luciferin jẹ sobusitireti fun adenosine triphosphate (ATP) -igbẹkẹle awọn aati bioluminescence.Idahun bioluminescence ti luciferin / luciferase ni a lo nigbagbogbo fun wiwa ATP, awọn metabolites ti o le yipada si ATP (bii AMP, ADP, cAMP), ati awọn enzymu ti o le ṣe ipilẹṣẹ ATP (bii creatine kinase, ati bẹbẹ lọ), nitorinaa. iṣesi bioluminescence le ṣee lo.Kan si wiwa ti ọpọlọpọ awọn ohun elo ti ibi.
Iṣẹ iṣe ti Ẹjẹ: D-Luciferin (Firefly luciferin) jẹ sobusitireti olokiki fun bioluminescence ni iwaju ATP fun lilo ninu aworan bioluminescence ti o da lori luciferase ati ibojuwo ti o da lori sẹẹli.
Awọn ẹkọ in vitro: D-luciferin fesi pẹlu luciferase, ATP ati atẹgun lati tan ina, eyiti a rii nipasẹ fiimu fọto ti o ni itara lati wo awọn ajẹsara ti o ni ipilẹ alkaline phosphatase.
Ninu awọn ẹkọ vivo: lilo awọn sobusitireti D-luciferin ati firefly luciferase ṣe itọju awọn ibaraẹnisọrọ ajẹsara tumor-host ni awoṣe asin ajẹsara pẹlu akàn ovarian, nitori awọn eto bioluminescence jẹ ifarabalẹ si idagbasoke tumo ju awọn ilana ere iwuwo ara lọ.