4-Nitrophenyl-alpha-D-galactopyranoside CAS:7493-95-0 99%
| Nọmba katalogi | XD900018 |
| Orukọ ọja | 4-Nitrophenyl-alpha-D-galactopyranoside |
| CAS | 7493-95-0 |
| Ilana molikula | C12H15NO8 |
| Òṣuwọn Molikula | 301.25 |
| Awọn alaye ipamọ | -15 si -20 °C |
| Ti irẹpọ Owo idiyele koodu | 29400000 |
Ọja Specification
| Ifarahan | Funfun to Orange to Green lulú to gara |
| Ayẹwo | 99% |
4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) jẹ sobusitireti atọwọda ti 4-nitrophenyl (pNP) glycopyranoside fun wiwa ti iṣẹ α-galactosidase.Iye pNP ti a tu silẹ ti pọ si ni pataki nigbati 4-Nitrophenyl α-D-galactpyranoside jẹ lilo bi sobusitireti.
Lilo4-nitorophenol (pNP) -glycopyranosidesaspseudosubstrates, theamountofliberatedpNPfromthe4-Nitrophenyla-D-galactopyranosideis pataki ti o ga julọ lati inu theotherpNP-glycopyranosidesatpH4.0whereasnoactivity wasde7. yl (pNP) glycopyranosidesasartificial substrates fihan pe o ni ominira ti galactoseresidues lati4-Nitrophenyla-D-galactopyranosidemainlyatpH4.0, iethepHlevel ibi ti o ti wa ni ipalọlọ ti dinku pupọ julọ.
Sobusitireti ti α-D-galactosidase (α-D-Galactosidase).Ti sopọ mọ E. coli lactose protein ti ngbe.Alabọde fun α-D-galactose.
Ọna iyara fun ṣiṣe iṣiro iṣẹ lytic ti awọn aṣoju antimicrobial lodi si iwukara ati elu ti ni idagbasoke.Ayẹwo naa da lori itusilẹ ti enzymu intracellular, maltase (alpha-glucosidase).Iṣẹ ṣiṣe maltase ti a tu silẹ ni a ṣe iwọn awọ nipasẹ iṣelọpọ p-nitrophenol lati p-nitrophenyl-alpha-D-glucopyranoside (PNPG).Iṣẹ-ṣiṣe lytic ti awọn agbo ogun antimicrobial oriṣiriṣi ni a wọn lodi si awọn sẹẹli iwukara tabi germinating spores ti filamentous elu.Iṣẹ ṣiṣe egboogi-iwukara Lytic le ṣee wa-ri laarin 20 min incubation ni 30 iwọn C lodi si Saccharomyces cerevisiae, Candida albicans, ati Cryptococcus neoformans.Iṣẹ ṣiṣe egboogi-olu ti Lytic han lẹhin awọn wakati 2 ti abeabo ni iwọn 30 C lodi si awọn spores germinating ti Aspergillus niger ati Botrytis cinerea.Gbogbo awọn sẹẹli boya iwukara tabi elu ko ṣe hydrolyze to PNPG laarin awọn wakati 3 ni iwọn 30 C lati mu iyipada awọ ti a rii.Iṣẹ-ṣiṣe Lytic ti awọn enzymu (fun apẹẹrẹ, Lyticase), awọn oogun aporo (fun apẹẹrẹ, Amphotericin B), ati igara ti n ṣe aporo-ara ti awọn kokoro arun ni a rii ni lilo idanwo naa.Ayẹwo egboogi-iwukara ti ni ibamu si ọna kika microtiter 96-daradara.Awọn igbelewọn mejeeji pese wiwa iyara, ifarabalẹ, ati iṣatunṣe ti iwukara iwukara lytic ati iṣẹ ṣiṣe egboogi-olu.
