![3- [(3- Cholanidopropyl) dimethylamonio] -1 -propanesulfonate Cas: 75621-03-3 98% Aworan ti a ṣe afihan](http://cdn.globalso.com/xdbiochems/75621-03-3.jpg)
3- [(3- Cholanidopropyl) dimethylamonio] -1 -propanesulfonate Cas: 75621-03-3 98%
| Nọmba katalogi | XD90075 |
| Orukọ ọja | 3-[(3-Cholanidopropyl) dimethylamonio]-1-propanesulfonate. |
| CAS | 75621-03-3 |
| Ilana molikula | C32H58N2O7S |
| Òṣuwọn Molikula | 614.88 |
| Awọn alaye ipamọ | Ibaramu |
| Ni ibamu Owo idiyele koodu | 29242970 |
Ọja Specification
| IR | Afiwera si itọkasi |
| Isonu lori Gbigbe | <2% |
| TLC | Aami kan |
| Nitrojiini | 4.46 - 4.65 |
| Ifarahan | funfun lulú |
| Solubility (10% ni H2O) | Ko o ati awọ |
| Iṣeṣe (0.1M ninu H2O) | <10us/cm |
| Ayẹwo HPLC | > 98% |
| UV (5% ni H2O) A290nm | <0.5 |
| UV (5% ni H2O) A400nm | <0.02 |
| Akoonu Solvent (nipasẹ GC) Dimethylformamide | <2.0% |
3-[3- (cholamidopropyl) dimethylamino] propanesulfonic acid lilo iyọ inu ati ọna iṣelọpọ
Iṣẹ iṣe ti Ẹmi: CHAPS, itọsẹ ti Cholicacid, jẹ ohun-ọgbẹ zwitterionic ti o solubilizes awọn ọlọjẹ awọ ara.A lo CHAPS lati ṣe iduroṣinṣin ọpọlọpọ awọn eka amuaradagba-DNA ati pe o le ṣe idaduro iṣẹ ṣiṣe biokemika ti awọn ọlọjẹ ni ojutu.
Nlo: CHAPS jẹ ifọṣọ zwitterionic ti kii ṣe denaturing fun biochemistry awo ara.Le ṣee lo lati solubilize awọn ọlọjẹ ara ilu ati dabaru awọn ibaraẹnisọrọ amuaradagba-amuaradagba.CHAPS ni iwuwo molikula micellar kekere (6,150) ati ifọkansi micelle to ṣe pataki (6-10 mM) ati pe o le yọkuro kuro ninu ayẹwo nipasẹ ṣiṣe itọju ara.O tun dara fun awọn ọlọjẹ solubilizing ni idojukọ isoelectric ati 2D electrophoresis.CHAPS jẹ lilo nigbagbogbo ni abinibi (ọfẹ urea) isoelectric isoelectric ati pe a ti rii lati funni ni ipinnu to dara julọ fun awọn igbaradi subcellular kan ati awọn ọlọjẹ ọgbin.Ni deede awọn ifọkansi laarin 2-4% (w/v) ni a lo fun awọn gels idojukọ isoelectric.
Nlo: Detergent zwitterionic ti kii ṣe denaturing fun biochemistry membran, eyiti o le ṣee lo lati solubilize awọn ọlọjẹ awọ ara ati fa awọn ibaraẹnisọrọ amuaradagba-amuaradagba duro;tun dara fun awọn ọlọjẹ solubilizing ni idojukọ isoelectric ati electrophoresis onisẹpo meji;ti a lo nigbagbogbo ni Non-denaturing (ko si urea) isoelectric fojusi;RNA ati DNA isediwon, ko si RNase ati DNA aṣayan iṣẹ-ṣiṣe
Nlo: Amphoteric surfactant, CHAPS jẹ detergent zwitterionic ti kii ṣe denaturing, protein lysate fun solubilizing awọn ọlọjẹ awọ ara ati awọn ibaraenisepo irọlẹ laarin awọn ọlọjẹ ati awọn ọlọjẹ.
