Bicyclo isomeric meji [4.1.0] heptane analogues ti glycosidase inhibitor galacto-validamine, (1R *,2S,3S,4S,5S,6S*) -5-amino-1- (hydroxymethyl) bicyclo[4.1.0] heptane -2,3,4-triol, ni a ti ṣajọpọ ni awọn igbesẹ 13 lati 2,3,4,6-tetra-O-benzyl-D-galactose.Awọn iṣẹ inhibitory ti awọn amines meji ti o ni ihamọ conformationally, ati awọn acetamides ti o baamu, ni iwọn lodi si awọn enzymu alpha-galactosidase iṣowo lati ewa kofi ati E. coli.Iṣẹ ṣiṣe ti idile glycosyl hydrolase GH27 henensiamu (ewa kofi) ni idinamọ ni idije nipasẹ 1R,6S-amine (7), ibaraenisepo abuda ti o jẹ ifihan nipasẹ iye K (i) ti 0.541 microM.GH36 E. coli alpha-galactosidase ṣe afihan ibaraenisepo abuda alailagbara pupọ pẹlu 1R,6S-amine (IC(50)= 80 microM).Diastereomeric 1S,6R-amine (9) ti a so ni ailera si awọn galactosidases mejeeji, (ewa kofi, IC (50) = 286 microM) ati (E. coli, IC (50) = 2.46 mM).
