Tris maleate Cas: 72200-76-1 99.5% White crystalline lulú
Nọmba katalogi | XD90057 |
Orukọ ọja | Tris maleate |
CAS | 72200-76-1 |
Ilana molikula | C4H11NO3 · C4H4O4 |
Òṣuwọn Molikula | 237.21 |
Awọn alaye ipamọ | Ibaramu |
Ti irẹpọ Owo idiyele koodu | 29221985 |
Ọja Specification
Ifarahan | Funfun okuta lulú |
Ayẹwo | 99.5% |
Cocrystalization ti lysyl-tRNA synthetase lati Thermus thermophilus pẹlu awọn oniwe-cognate tRNAlys ati pẹlu Escherichia coli tRNAlys.
Lysyl-tRNA synthetase lati Thermus thermophilus ti jẹ cocrystallized pẹlu boya cognate tRNAlys tabi Escherichia coli tRNAlys ni lilo ammonium sulfate bi itusilẹ.Awọn kirisita dagba lati awọn ojutu ti o ni 1:2.5 stoichiometry ti synthetase dimer si tRNA ni 18-22% ammonium sulfate ni 50 mM Tris-maleate buffer ni pH 7.5.Awọn eka mejeeji jẹ onigun prismatic, awọn kirisita tetragonal pẹlu awọn paramita sẹẹli ti o jọra pupọ (a = b = 233 A, c = 119 A) ati diffract si o kere ju 2.7 A ipinnu.Sibẹsibẹ homocomplex jẹ ti ẹgbẹ aaye P42 (1) 2 ati heterocomplex ti ẹgbẹ aaye I422.
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