Awọn ọja

Reagent olomi-omi fun ipinnu photometric enzymatic ti hydrogen peroxide.Awọn isọdọtun Trinder tuntun jẹ awọn itọsẹ aniline ti o yo omi pupọ ti o jẹ lilo pupọ ni awọn idanwo iwadii ati awọn idanwo biokemika.Awọn anfani pupọ lo wa lori awọn reagents chromogenic ti aṣa ni ipinnu colorimetric ti iṣẹ ṣiṣe hydrogen peroxide.Awọn isọdọtun Trinder tuntun jẹ iduroṣinṣin to lati ṣee lo ninu ojutu mejeeji ati awọn eto wiwa opo gigun ti esiperimenta.Ni iwaju hydrogen peroxide ati peroxidase, aramada Trinder's reagent ti han lati fesi pẹlu 4-aminoantipyrine (4-AA) tabi 3-methylbenzothiazole sulfonehydrazone (MBTH) lakoko iṣesi idapọ oxidative.Fọọmu pupọ iduroṣinṣin aro tabi awọn awọ buluu.Imukuro molar ti awọ pọ pẹlu MBTH jẹ awọn akoko 1.5-2 ti o ga ju ti awọ pọ pẹlu 4-AA;sibẹsibẹ, ojutu 4-AA jẹ iduroṣinṣin diẹ sii ju ojutu MBTH lọ.Sobusitireti jẹ oxidized enzymatically nipasẹ oxidase rẹ lati gbejade hydrogen peroxide.Ifojusi hydrogen peroxide ni ibamu si ifọkansi sobusitireti.Nitorinaa, iye ti sobusitireti le jẹ ipinnu nipasẹ idagbasoke awọ ti iṣesi idapọ oxidative.Glukosi, oti, acyl-CoA ati idaabobo awọ le ṣee lo lati ṣe awari awọn sobusitireti wọnyẹn papọ si aramada Trinder's reagent ati 4-AA.Awọn reagents Trinder 10 tuntun wa.Lara awọn atunṣe Trinder tuntun, TOOS jẹ eyiti a lo julọ.Bibẹẹkọ, fun sobusitireti kan pato, idanwo awọn kilasi oriṣiriṣi ti awọn reagents aramada Trinder jẹ pataki lati ṣe agbekalẹ eto wiwa ti o dara julọ.

Reagent olomi-omi fun ipinnu photometric enzymatic ti hydrogen peroxide.Awọn isọdọtun Trinder tuntun jẹ awọn itọsẹ aniline ti o yo omi pupọ ti o jẹ lilo pupọ ni awọn idanwo iwadii ati awọn idanwo biokemika.Awọn anfani pupọ lo wa lori awọn reagents chromogenic ti aṣa ni ipinnu colorimetric ti iṣẹ ṣiṣe hydrogen peroxide.Awọn isọdọtun Trinder tuntun jẹ iduroṣinṣin to lati ṣee lo ninu ojutu mejeeji ati awọn eto wiwa opo gigun ti esiperimenta.Ni iwaju hydrogen peroxide ati peroxidase, aramada Trinder's reagent ti han lati fesi pẹlu 4-aminoantipyrine (4-AA) tabi 3-methylbenzothiazole sulfonehydrazone (MBTH) lakoko iṣesi idapọ oxidative.Fọọmu pupọ iduroṣinṣin aro tabi awọn awọ buluu.Imukuro molar ti awọ pọ pẹlu MBTH jẹ awọn akoko 1.5-2 ti o ga ju ti awọ pọ pẹlu 4-AA;sibẹsibẹ, ojutu 4-AA jẹ iduroṣinṣin diẹ sii ju ojutu MBTH lọ.Sobusitireti jẹ oxidized enzymatically nipasẹ oxidase rẹ lati gbejade hydrogen peroxide.Ifojusi hydrogen peroxide ni ibamu si ifọkansi sobusitireti.Nitorinaa, iye ti sobusitireti le jẹ ipinnu nipasẹ idagbasoke awọ ti iṣesi idapọ oxidative.Glukosi, oti, acyl-CoA ati idaabobo awọ le ṣee lo lati ṣe awari awọn sobusitireti wọnyẹn papọ si aramada Trinder's reagent ati 4-AA.Awọn reagents Trinder 10 tuntun wa.Lara awọn atunṣe Trinder tuntun, TOOS jẹ eyiti a lo julọ.Bibẹẹkọ, fun sobusitireti kan pato, idanwo awọn kilasi oriṣiriṣi ti awọn reagents aramada Trinder jẹ pataki lati ṣe agbekalẹ eto wiwa ti o dara julọ.