SYBR GREEN I Nucleic Acid Gel Stain Cas: 163795-75-3 99% Ri to
| Nọmba katalogi | XD90236 |
| Orukọ ọja | SYBR GREEN Mo Nucleic Acid jeli Awọ |
| CAS | 163795-75-3 |
| Ilana molikula | C32H37N4S |
| Òṣuwọn Molikula | 509.728 |
| Awọn alaye ipamọ | -20°C |
Ọja Specification
| Ifarahan | funfun lulú |
| Ayẹwo | 99% |
Sitometry ṣiṣan jẹ ọna idi kan fun ṣiṣe ifọnọhan awọn igbeyẹwo ifamọ antimalarial in vitro pẹlu agbara jijẹ fun ohun elo ni awọn aaye aaye.A ṣe ayẹwo ifaragba in vitro si awọn oogun egboogi-iba meje fun 40 alabapade P. falciparum aaye ti o ya sọtọ nipasẹ ọna cytometry ṣiṣan (FCM), ELISA ti o ni awọ-ara LDH: DELI), ati itupalẹ ifaworanhan microscopic boṣewa ti idagbasoke.Fun FCM, awọn idanwo 184/280 (66%) pade awọn ibeere gbigba itupale, ni akawe si 166/280 (59%) fun DELI.Adehun ti o dara wa laarin FCM ati maikirosikopu, lakoko ti DELI ṣe itara lati gbejade awọn iwọn idawọle idaji ti o ga julọ (IC50s) ju FCM, pẹlu irẹjẹ gbogbogbo ti 2.2-agbo (lafiwe Bland-Altman).Awọn iye fun artesunate ati dihydroartemisinin ni o kan julọ.Awọn ilọsiwaju paradoxical ni ifihan ni awọn ifọkansi giga pupọ ti mefloquine ati awọn agbo ogun ti o ni ibatan jẹ aami diẹ sii pẹlu idanwo DELI, ni iyanju pe awọn ipa ibi-afẹde lori iṣelọpọ LDH le jẹ iduro.Pipadanu ifihan FCM nitori isọdọtun tabi idagbasoke ti o lọra ni a ṣe akiyesi ni nọmba kekere ti awọn ayẹwo.Awọn abajade wọnyi faagun iṣẹ iṣaaju lori lilo cytometry ṣiṣan lati pinnu ailagbara antimalarial ni awọn ofin ti nọmba awọn ayẹwo, ibiti awọn oogun, ati lafiwe pẹlu awọn ọna miiran.
