Streptomycin sulfate CAS: 3810-74-0 Funfun lulú
| Nọmba katalogi | XD90361 |
| Orukọ ọja | Streptomycin sulfate |
| CAS | 3810-74-0 |
| Ilana molikula | (C21H39N7O12) 2 · 3H2SO4 |
| Òṣuwọn Molikula | 1457.39 |
| Awọn alaye ipamọ | 2 si 8 °C |
| Ti irẹpọ Owo idiyele koodu | 29412080 |
Ọja Specification
| Ipele | USP36 / EP8.0 |
| pH | 4.5-7.0 |
| Isonu lori Gbigbe | ≤7.0% |
| Ayẹwo | ≥720IU/MG |
| Sulfate | 18.0 ~ 21.5% |
| Ifarahan | Funfun tabi fere funfun lulú, lalailopinpin tiotuka ninu omi, fere insoluble ni ethanol. |
| kẹmika kẹmika | 0.3% |
| Streptomycin B | ≤3.0% |
| Idanwo Colorimetric | ≥90.0% |
| Sulfuric Acid | ≤1.0% |
| Akoonu iṣuu soda sulphite ti o ni idojukọ | ≤0.4% |
Bi o ti jẹ pe etiology ti disiki intervertebral degeneration ti ko ni oye, ọna kan lati dena ilana yii le jẹ lati dẹkun apoptosis.Ninu iwadi lọwọlọwọ, awọn ipa anti-apoptotic ti carboxymethylated chitosan (CMCS) ninu awọn sẹẹli nucleus pulposus (NP) ni a ṣe iwadii pẹlu ifọkansi lati jẹki iwalaaye sẹẹli disiki.Awọn sẹẹli NP Rat ti ya sọtọ ati gbin ni fitiro, ati hydrogen peroxide (H2O2) ni a lo lati kọ awoṣe apoptosis sẹẹli NP.A ṣe ayẹwo ṣiṣeeṣe sẹẹli pẹlu ohun elo kika sẹẹli kan-8.Iwọn ti awọn sẹẹli apoptotic jẹ iwadi nipasẹ annexin V-fluorescein isothiocyanate (FITC) ati propidium iodide (PI) itupalẹ abawọn ilọpo meji, ati pe mofoloji jẹ akiyesi nipasẹ Hoechst 33342 abawọn.Agbara awọ ara mitochondrial ti awọn sẹẹli NP ni a ṣe ayẹwo nipasẹ abawọn fluorescence rhodamine 123.Iyipada transcription (RT) ‑ pipo polymerase chain reaction (qPCR) ni a ṣe lati wiwọn awọn ipele mRNA ti inducible nitric oxide synthase (iNOS), caspase‑3, lymphoma B-cell (Bcl)‑2, iru II collagen ati aggrecan.A ṣe itupalẹ abawọn iwo-oorun lati ṣawari awọn ipele amuaradagba ti iNOS ati Bcl-2.Annexin V-FITC/PI ati Hoechst 33342 awọn abajade idoti fihan pe CMCS ni anfani lati ṣe idiwọ awọn sẹẹli NP lati apoptosis ni ọna ti o gbẹkẹle iwọn lilo.Abawọn Rhodamine 123 ṣe alaye pe CMCS dinku ailagbara ti awo awọ mitochondrial ninu awọn sẹẹli NP ti a ṣe itọju H2O2.Caspase-3 ti o dinku ati iṣẹ ṣiṣe Bcl-2 ti o pọ si ni a rii ni awọn sẹẹli NP ti CMCS ṣe itọju nipasẹ RT‑qPCR ati itupalẹ abawọn iwọ-oorun.CMCS tun ṣe igbega igbega ati itujade ti iru II collagen ati aggrecan ninu awọn sẹẹli NP ti a ṣe itọju H2O2.A ṣe afihan CMCS lati munadoko ni idilọwọ iku sẹẹli apoptotic ni vitro, ti n ṣe afihan awọn anfani ti o pọju ti ọna itọju ailera yii ni ṣiṣakoso degeneration disiki.
