Amuaradagba Kinase Afikun KT5823 CAS: 126643-37-6
| Nọmba katalogi | XD90398 |
| Orukọ ọja | Amuaradagba Kinase Supplement KT5823 |
| CAS | 126643-37-6 |
| Ilana molikula | C29H25N3O5 |
| Òṣuwọn Molikula | 495.53 |
| Awọn alaye ipamọ | 2 si 8 °C |
| Ti irẹpọ Owo idiyele koodu | 29349990 |
Ọja Specification
| Ifarahan | funfun lulú |
Awọn olugba P2X3 ATP-gated jẹ awọn transducers pataki ti awọn aapọn nociceptive ati pe o fẹrẹ ṣafihan iyasọtọ nipasẹ awọn neuronu ganglion sensory.Ninu ganglion trigeminal Asin (TG), iṣẹ olugba P2X3 ti ni ilọsiwaju lairotẹlẹ nipasẹ bulọọki elegbogi ti natriuretic peptide receptor-A (NPR-A), ti n ṣalaye ipa ipanilara ti o pọju ti awọn peptides natriuretic endogenous ni nociception nipasẹ awọn olugba P2X3.Aini iyipada ninu ikosile amuaradagba P2X3 tọkasi modulation eka kan eyiti awọn ọna ṣiṣe fun isọdọtun iṣẹ olugba P2X3 ko ṣe akiyesi.Lati ṣe alaye ilana yii ni awọn aṣa TG Asin, a tẹ ami ifihan NPR-A kuro pẹlu boya siRNA ti agonist endogenous BNP, tabi NPR-A blocker anantin.Nitorinaa, a ṣe iwadii awọn ayipada ninu pinpin olugba P2X3 ni kompaktimenti membran raft lipid, ipo phosphorylation wọn, bakanna bi iṣẹ wọn pẹlu patch clamping.Idaduro ibẹrẹ ti aibalẹ P2X3 jẹ ilana kan fun imudara ti a fa anantin ti iṣẹ P2X3.Anant ninu ohun elo fa atunkọ olugba P2X3 ayanfẹ si yara raft ọra ati idinku P2X3 serine phosphorylation, awọn iṣẹlẹ meji ti ko ni igbẹkẹle.Inhibitor ti cGMP-ti o gbẹkẹle protein kinase ati siRNA-mediated knockdown ti BNP mimicked ipa ti anantin.A ṣe afihan pe ninu awọn eku trigeminal neurons endogenous BNP ṣe lori awọn olugba NPR-A lati pinnu ibanujẹ idawọle ti iṣẹ olugba P2X3.Idena Tonic ti iṣẹ-ṣiṣe olugba P2X3 nipasẹ awọn ọna BNP/NPR-A/PKG waye nipasẹ awọn ọna ṣiṣe ọtọtọ meji: P2X3 serine phosphorylation ati atunkọ olugba si awọn ẹya ara ilu ti kii-raft.Ilana aramada yii ti iṣakoso olugba le jẹ ibi-afẹde fun awọn ẹkọ iwaju ti o pinnu lati dinku iṣẹ-ṣiṣe olugba P2X3 dysregulated ni irora onibaje.
