PMSF Cas: 329-98-6 98.0% funfun kristali lulú Phenylmethanesulfonyl fluoride (PMSF)
| Nọmba katalogi | XD90250 |
| Orukọ ọja | Phenylmethanesulfonyl fluoride (PMSF) |
| CAS | 329-98-6 |
| Ilana molikula | C7H7FO2S |
| Òṣuwọn Molikula | Ọdun 174.1927 |
| Awọn alaye ipamọ | Ibaramu |
| Ti irẹpọ Owo idiyele koodu | 29049900 |
Ọja Specification
| Ayẹwo | ≥98.0% HPLC |
| Ifarahan | funfun kirisita lulú |
PMSF jẹ inhibitor serine/cysteine protease ti kii ṣe iyipada ti o wọpọ ni igbaradi ti awọn lysates sẹẹli.
Awọn ẹkọ in vitro: PMSF (2 mM) ṣe idiwọ ikojọpọ inositol fosifeti ti carbachol-stimulated nipasẹ 15% -19% nikan ni iwaju Li +.Idinamọ ti iyipada phosphoinotide nipasẹ PMSF jẹ nitori ọkan tabi diẹ ẹ sii awọn igbesẹ ti o tẹle didenukole ti phosphoinotides [1].PMSF ṣe idiwọ acylation ti awọn iṣẹku inositol ti awọn agbedemeji GPI ninu ẹjẹ ti T. brucei.PMSF ṣe idiwọ dida glycolipid C ṣugbọn kii ṣe atunṣe acid fatty ni fitiro.PMSF ṣe idiwọ afikun ti GPI acylation ati ethanolamine phosphatase ni awọn trypanosomes procyclic, ṣugbọn kii ṣe ninu awọn sẹẹli Hela [2].
Ninu awọn ẹkọ vivo: PMSF (0.1 mL / 10 g b.wt, ip) ṣe agbejade antinociception, bi a ṣe fihan nipasẹ iwọn-idahun iwọn lilo% MPE ni igbelewọn lairi iru-flick, ṣugbọn kuna lati gbejade idinamọ iwọn-idahun iwọn lilo ti o han gbangba.Awọn eku ti o gba abẹrẹ intraperitoneal ti PMSF ṣe afihan awọn ipa cannabinoid pẹlu antinociception, hypothermia ati ailagbara pẹlu awọn iye ED50 ti 86, 224 ati 206 mg/kg, lẹsẹsẹ.PMSF (30 mg / kg) pretreatment mu awọn ipa ti anandamide ṣe lori awọn idahun flick iru (antinociception), iṣẹ-ṣiṣe locomotor ati iṣipopada nipasẹ 5-fold, 10-fold and 8-fold, lẹsẹsẹ [3].
Awọn adanwo ẹranko: Awọn eku ICR ọkunrin ti wọn ṣe iwọn 18 si 25 g ni a lo ninu idanwo naa.PMSF ni tituka ni epo Sesame ati ti a nṣakoso intraperitoneally ni iwọn didun 0.1 mL/10 g b.wt.Ṣe abojuto PMSF nigbagbogbo ni iṣẹju mẹwa 10 ṣaaju anandamide iṣan iṣan tabi abẹrẹ ọkọ.Awọn eku ni acclimated si yara igbelewọn ni alẹ moju laisi idilọwọ ounjẹ tabi omi.Lẹhin anandamide inu iṣọn-ẹjẹ tabi iṣakoso ọkọ, ẹranko kọọkan ni a ṣe ayẹwo bi atẹle: Awọn iṣẹju 5 fun awọn idahun iru-flick (antinociceptive) ati awọn iṣẹju 5 si 15 fun iṣẹ-ṣiṣe lẹẹkọkan (motor);tabi 5 iṣẹju fun mojuto (rectal) otutu ati oruka Immobilization (catalepsy) fun 5 to 10 iṣẹju.
