Awọn ọja

Idinamọ ti β-carbonic anhydrases (CAs, EC 4.2.1.1) lati awọn pathogenic elu Cryptococcus neoformans (Can2) ati Candida albicans (Nce103) pẹlu lẹsẹsẹ 25 ẹka aliphatic ati aromatic carboxylates ti ṣe iwadii.Awọn isoforms eniyan hCA I ati II tun wa ninu iwadi fun lafiwe.Aliphatic carboxylates jẹ gbogbo millimolar hCA I ati II inhibitors ati kekere micromolar/submicromolar β-CA inhibitors.Awọn carboxylates aromatic jẹ awọn inhibitors micromolar ti awọn enzymu mẹrin ṣugbọn diẹ ninu wọn ṣe afihan iṣẹ ṣiṣe nanomolar kekere lodi si awọn enzymu pathogenic olu.4-Hydroxy- ati 4-methoxy-benzoate ṣe idiwọ Can2 pẹlu K (I) ti 9.5-9.9 nM.Awọn esters methyl, hydroxamates, hydrazides ati awọn carboxamides ti diẹ ninu awọn itọsẹ wọnyi tun jẹ awọn inhibitors ti o munadoko ti α- ati β-CA ti ṣe iwadii nibi.

Parabens wa laarin awọn ohun itọju igbagbogbo ti a lo nigbagbogbo lati ṣe idiwọ idagbasoke makirobia ati fa igbesi aye selifu ti ọpọlọpọ awọn ọja olumulo.Idi ti iwadii lọwọlọwọ ni lati ni oye si iṣelọpọ ti parabens ninu awọn sẹẹli alakan igbaya (MCF7) nitori wọn ti ṣe afihan iṣẹ ṣiṣe estrogenic si awọn sẹẹli wọnyi ati pe a ti rii ni awọn iṣan akàn igbaya.Majele ti parabens si awọn sẹẹli MCF7 ti pinnu nipa lilo awọn idanwo MTT.Hydrolysis ti methyl-, butyl ati benzyl-paraben si p-hydroxybenzoic acid ni a ṣe atupale ni awọn sẹẹli MCF7 ti gbin ati ni awọn homogenates cellular.Glucuronidation ati sulfoconjugation ni a ṣe iwadi ni MCF7 homogenates, ati awọn parabens ni a ṣe atupale nipasẹ HPLC.Methyl-paraben jẹ majele ti o kere ju butyl ati benzyl-paraben lọ.Parabens jẹ iduroṣinṣin patapata ni awọn homogenates MCF7 lakoko ti p-nitrophenyl acetate, iru sobusitireti kan, ti ṣe hydrolysis.Awọn homogenates sẹẹli MCF7 ko ṣe afihan glucuronidation ati awọn iṣẹ sulfoconjugation si awọn parabens.Iduroṣinṣin ti o ga julọ ti parabens le ṣe alaye ikojọpọ wọn ninu àsopọ ọgbẹ igbaya.