Nigrosine (CI 50420) (Acid Black 2) CAS: 8005-03-6 Black Flash Saccharoid
| Nọmba katalogi | XD90456 |
| Orukọ ọja | Nigrosine (CI 50420) (Acid Black 2) |
| CAS | 8005-03-6 |
| Ilana molikula | - |
| Òṣuwọn Molikula | - |
| Awọn alaye ipamọ | 2 si 8 °C |
| Ti irẹpọ Owo idiyele koodu | 32129000 |
Ọja Specification
| Ifarahan | Black Flash Saccharoid |
| Eeru | <1.7% |
| Agbara | 100% ± 3 |
| Ọrinrin | <8% |
Idanwo iranran ti o rọrun ni idagbasoke, eyiti o fun laaye ni iwọn ti microalbuminuria.A ṣe ayẹwo ni ibamu si awọn ilana ISO 15189. A ti rii ito lori awọn ila acetate cellulose ati abariwon nipa lilo awọn awọ mimu amuaradagba ti o yatọ (nigrosin, Coomassie Blue R-250, amido dudu).Iwọn awọ ti awọn aaye ti o ni abawọn ni a ṣe iwọn nipa lilo Kodak Image 450 station. Ifamọ itupalẹ ti ọna orisun Coomassie Blue (18 mg / L) dara ju ti nigrosin (50 mg / L) tabi amido dudu (100 mg / L) ) awọn ọna orisun.Olusọdipúpọ laarin-ṣiṣe ti iyatọ (CV) ati laarin-ṣiṣe CV ti ayẹwo buluu Coomassie jẹ, lẹsẹsẹ, 8.4% ati 9.7% (50 mg/L), ati 3% ati 4.5% (400 mg/L).Fun nigrosin, awọn data wọnyi jẹ, lẹsẹsẹ, 8.4 ati 9.4 (50 mg/L), ati 3.4 ati 6.4% (400 mg/L).Coomassie Blue ṣe afihan yiyan abuda yiyan si ọna albumin.Ọna naa ni a rii lati jẹ laini laarin 20 ati 600 mg / L.Ibaṣepọ to dara (r2 = 0.89) ni a gba laarin orisun Coomassie Blue ati awọn wiwọn ajẹsara.albumin ti ko ni ajẹsara (ti a pese sile nipasẹ itọju protease) ni a le rii nipasẹ idanwo iranran, eyiti o funni ni anfani ti ọna dipo awọn idanwo ajẹsara.Ammonium sulphate ojoriro le siwaju sii mu awọn pato ti awọn assay nipa yiyo awọn ipa ti free ina chains.Awọn apejuwe ọna ti o rọrun pupọ ati ki o lalailopinpin olowo poku, eyi ti o mu ki o ni agbara ti o baamu fun awọn eto ibojuwo, paapaa ni awọn orilẹ-ede agbaye kẹta.
