Dimethyl ofeefee CAS: 60-11-7 Yellow to Orange to brown powder
| Nọmba katalogi | XD90467 |
| Orukọ ọja | Dimethyl ofeefee |
| CAS | 60-11-7 |
| Ilana molikula | C14H15N3 |
| Òṣuwọn Molikula | 225-29 |
| Awọn alaye ipamọ | Ibaramu |
| Ti irẹpọ Owo idiyele koodu | 29270000 |
Ọja Specification
| Ojuami Iyo | 105-116 Deg C |
| Ifarahan | Yellow to Orange to brown lulú |
| Ayẹwo | 99% |
| Isonu lori Gbigbe | <1.0% |
| Agbara | 98.67% |
| Iyipada Awọ | Pupa @ pH 2.9, Yellow @ pH 4.9 |
| eeru sulfated | 0.4% |
| Lama Max | 400nm (ni methanol) |
Topology awo ilu ti agbegbe ikanni colicin E1 ni a ṣe iwadi nipasẹ gbigbe agbara resonance fluorescence (FRET).FRET naa kan amino acid fluorescent ti a fi aami si jiini (coumarin) gẹgẹbi oluranlọwọ ati iyọkuro cysteine ti a yan ti a yan pẹlu DABMI (4- (dimethylamino) phenylazophenyl-4'-maleimide) gẹgẹbi olugba FRET.Aloku coumarin fluorescent ni a dapọ si amuaradagba nipasẹ ọna meji tRNA/aminoacyl-tRNA synthetase orthogonal ti o gba laaye isọpọ yiyan sinu eyikeyi aaye laarin aaye ikanni colicin.Iyatọ kọọkan ni idaduro iyipada (TAG) fun isọdọkan coumarin ati iyipada cysteine (TGT) fun asomọ DABMI.Awọn ijinna interhelical mẹfa laarin awọn helices 1-6 ni a pinnu nipa lilo itupalẹ FRET fun awọn ipinlẹ ti o soluble ati awọ-ara.Awọn data FRET ṣe afihan awọn ayipada nla ni awọn ijinna interhelical laarin awọn helices 3-6 lori ẹgbẹ membran ti n pese oye tuntun sinu eto-ipin awo-ara ti agbegbe ikanni.Ni gbogbogbo, awọn iṣẹ ṣiṣe interhelical coumarin-DABMI FRET dinku lori isopọmọ awo awọ, ti o kọ sori awoṣe agboorun fun ikanni colicin.Awoṣe agọ kan fun ipo pipade ti aaye ikanni jẹ idagbasoke da lori lọwọlọwọ ati data FRET ti a tẹjade tẹlẹ.Awoṣe naa ni imọran iṣeto ipin ti awọn helices 1-7 ni itọsọna aago lati ẹgbẹ extracellular ati ẹgbẹ interfacial membran ti helices 1, 6, 7, ati 10 ni ayika irun transmembrane aringbungbun ti a ṣẹda nipasẹ helices 8 ati 9.
![3,3′,5,5′-Tetramethyl-[1,1'-biphenyl]-4,4′-diamine Cas:54827-17-7 99%](http://cdn.globalso.com/xdbiochems/54827-17-71.jpg)
