BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine iyọ Cas:6578-06-9 99% Funfun si ina brown lulú
Nọmba katalogi | XD90137 |
Orukọ ọja | BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine iyọ. |
CAS | 6578-06-9 |
Ilana molikula | C8H5BrClNO4P·C7H10N |
Òṣuwọn Molikula | 433.62 |
Awọn alaye ipamọ | -15 si -20 °C |
Ti irẹpọ Owo idiyele koodu | 29339980 |
Ọja Specification
Ifarahan | Funfun / ina brown lulú |
Asay | ≥ 99% |
Ojuami yo | 194-195ºC |
Oju omi farabale | 580.2°C ni 760 mmHg |
oju filaṣi | 304.7°C |
Solubility | DMF: 20 mg/ml |
Solubility | tiotuka ni dimethylformamide, insoluble ninu omi. |
Ifamọ | Ni imọlara si ina, ifarabalẹ si afẹfẹ, ati ifarabalẹ si ooru |
Iṣẹ ṣiṣe ti ara: BCIP (iyọ BCIP p-toluidine; iyọ X-phosphate p-toluidine) jẹ sobusitireti chromogenic fun wiwa awọ-ara ti iṣẹ ṣiṣe phosphatase ipilẹ.
Awọn majele Shiga (Stxs) jẹ iṣelọpọ nipasẹ enterohemorrhagic Escherichia coli (EHEC), eyiti o fa awọn akoran eniyan pẹlu abajade apaniyan nigbagbogbo.Awọn laini sẹẹli Vero, ti o jade lati kidinrin obo alawọ ewe Afirika, ṣe aṣoju boṣewa goolu fun ṣiṣe ipinnu awọn ipa cytotoxic ti Stxs.Pelu lilo agbaye wọn, imọ nipa awọn ẹya gangan ti Stx receptor glycosphingolipids (GSLs) ati apejọ wọn ni awọn rafts ọra ko dara.Nibi a ṣe afihan igbekale igbekalẹ igbekalẹ ti awọn GSL olugba Stx ati pinpin wọn si awọn membran-sooro detergent (DRMs), eyiti a pese sile lati awọn sẹẹli Vero-B4 ati lo bi awọn deede raft ọra.A ṣe idanimọ globotriaosylceramide (Gb3Cer) ati globotetraosylceramide (Gb4Cer) gẹgẹbi awọn olugba GSL fun Stx1a, Stx2a, ati Stx2e subtypes nipa lilo wiwa apọju TLC ni idapo pẹlu MS.Olugba Stx ti ko wọpọ, globopentaosylceramide (Gb5Cer, Galβ3GalNAcβ3Galα4Galβ4Glcβ1Cer), eyiti o jẹ idanimọ ni pataki (ni afikun si Gb3Cer ati Gb4Cer) nipasẹ Stx2e, jẹ ẹya ti iṣeto ni kikun.Awọn lipoforms ti awọn GSL olugba Stx ni a rii ni pataki awọn ohun elo ceramide ti o ni sphingosine (d18:1) ati C24: 0/C24: 1 tabi C16: 0 fatty acid.Pẹlupẹlu, iṣọpọ-iṣẹlẹ pẹlu awọn ami ami raft lipid, SM ati idaabobo awọ, ni awọn DRMs daba ajọṣepọ GSL pẹlu microdomains awo.Iwadi yii n pese ipilẹ fun wiwa siwaju si ipa iṣẹ ti awọn olugba Stx ti o ni nkan ṣe pẹlu raft raft fun ipalara ti majele ti awọn sẹẹli Vero-B4.