Awọn ọja

Chemifluorescence: Molecular luminol is a chemically fluorescent molecule ti o le ṣe iyipada si aminophthalic acid ipinle ti o ni itara ni iwaju awọn ohun elo hydrogen peroxide, eyiti o njade imọlẹ to lagbara.Hydrogen peroxide jẹ ọja ti ọpọlọpọ awọn aati biooxidative, nitorinaa o rọrun lati sopọ awọn aati biooxidative wọnyi pẹlu wiwadi fọto nipasẹ iṣafihan luminol.Fun apẹẹrẹ, iwadii glukosi oxidase / catalase le rii ifọkansi ti hydrogen peroxide tabi glukosi ninu apẹẹrẹ, ati akoko idahun jẹ 0.5s nikan (ọna agbara).Apapọ awọn nkan kemiluminescent pẹlu awọn aati ajẹsara, ati sisọ ifọkansi ti awọn paati ajẹsara ti a rii nipasẹ awọn aati ina, ni a pe ni imọ-ẹrọ immunoassay chemiluminescent.Ni ọdun 1976, Shmeder dabaa chemiluminescence immunoassay, ni lilo luminol-H202 ati itọsẹ ABEI rẹ gẹgẹbi eto ifihan ifarahan wiwa.Lọwọlọwọ, luminol ati awọn itọsẹ rẹ jẹ awọn ami-ami ti o wọpọ julọ ti a lo ni awọn ohun elo immunoassay chemiluminescence.Labẹ awọn ipo ipilẹ, catalyzed nipasẹ microperoxidase, nọmba nla ti awọn fọto le jẹ idasilẹ.Luminol le ṣee lo fun WesternBlot ti awọn apakokoro ti o ni aami HRP ati wiwa arabara acid nucleic ti awọn iwadii ti aami HRP.O ti wa ni tun lo ni igbalode odaran Otelemuye erin bloodstain.Apapọ ti o le ṣee lo bi ami ami kemiluminescent gbọdọ pade awọn ipo wọnyi: ikore kuatomu ti luminescence ga;awọn ohun-ini physicokemikali ibaamu eto ti o wa labẹ iwadi;Ihuwasi itanna rẹ jẹ abajade ti iṣesi ifoyina ti nkan luminescent;laarin iwọn ifọkansi ti a lo Ni inu ti kii ṣe majele si awọn ohun alumọni ti ngbe.Orisirisi awọn orisi ti chemiluminescence reagents ti wa ni commonly lo: acridine ester, kan ni opolopo lo chemiluminescence tracer, o jẹ a tricyclic Organic yellow, rọrun lati oxidize, ati awọn ifoyina lenu ko ni beere a ayase, ati tu photons ni 430nm;Luminol ati isoluminol ati awọn itọsẹ wọn jẹ awọn aṣoju chemiluminescent ti o dagba julọ.Ni ibẹrẹ ọdun 1964, Lakoko ti o royin lori chemiluminescence, luminol, isoluminol ati awọn itọsẹ wọn ni a lo fun kemiluminescence.Immunoassays ti ṣaṣeyọri awọn abajade to dara julọ.Wiwa ti itanna: gigun igbi fluorescence ti o dara julọ jẹ 400nm (iwari ti kemiluminescence ni 60mmK2S2O8, 100mmK2CO3, pH11.5 ojutu)

Chemiluminescence: Gẹgẹbi ọna igbelewọn ifura, kemiluminescence jẹ lilo pupọ lati ṣe awari awọn ipilẹṣẹ ọfẹ ati awọn metabolites ifaseyin ti a ṣejade ni awọn enzymu, awọn sẹẹli ati awọn oganisimu ti ibi.Imọlẹ ti njade nipasẹ awọn ọja ifoyina wọn ati awọn metabolites le ṣee lo pẹlu ọpọlọpọ awọn photometers.wiwa.Chemiluminescence jẹ lilo pupọ ni wiwa ati iwadii awọn antioxidants, gẹgẹbi awọn polyphenols, polysaccharides, flavonoids, ati anthraquinones, nitori ifamọ rẹ, iyara, iṣẹ ti o rọrun ati idiyele kekere.Awọn ọna ṣiṣe chemiluminescence ti o wọpọ fun ipinnu awọn anions superoxide pẹlu xanthine oxidase-luminol, pyrogallol-luminol ati dimethyl sulfoxide-luminol.Awọn tele jẹ ẹya enzymatic eto, ati awọn igbehin meji O ti wa ni a ti kii-enzymatic eto.Awọn ọna ṣiṣe kemiluminescence fun ipinnu ti awọn ipilẹṣẹ hydroxyl ni akọkọ pẹlu Ejò sulfate-iwukara (tabi awọn sẹẹli ọra inu egungun) -ascorbic acid-hydrogen peroxide, CuCl-H2O2-o-phenanthroline-carbonate buffer, Ejò sulfate-o-phenanthroline-ascorbic acid-hydrogen peroxide 5 chemiluminescence awọn ọna šiše, ferrous sulfate-luminol-hydrogen peroxide ati ferrous sulfate-luminol, gbogbo awọn mudani awọn Ayebaye Fenton lenu lati se ina hydroxyl radicals, ati ki o si kolu awọn luminescent oluranlowo lati se ina kemiluminescence, eyi ti o le ṣee lo lati wiwọn awọn yiyọ ti awọn ayokuro The aṣayan iṣẹ-ṣiṣe ti awọn ipilẹṣẹ atẹgun ifaseyin.Ọna idanwo: lo pyrogallol-luminol chemiluminescence system: luminol ti ṣe sinu 0.05mol/L ojutu ifọkansi 0.05mol/L NaOH ojutu, ti a fipamọ sinu ibi dudu, ati omi distilled meji ṣaaju lilo Dilute si ojutu 1mmol / L, lo 1mmol/ L HCl lati ṣe ojutu 0.01mol / L ti pyrogallol, tọju rẹ sinu firiji ni 4 ° C, ki o si dilute o pẹlu omi distilled meji si awọn akoko 16 6.25 × 10-4mol / L ojutu ṣaaju lilo.0.05mol/L pH10.2Na2CO3-NaHCO3 buffer (ti o ni 0.1mmol/LEDTA) ti pese sile ṣaaju lilo, a si dapọ pẹlu 1mmol/L luminol ni 2: 1 (ida iwọn didun) lati ṣe luminol ati carbonate buffer ṣaaju ki o to adalu adanwo.Lakoko wiwọn, fa awọn ayẹwo 10.0 μL ti awọn ifọkansi oriṣiriṣi (0, 0.08, 0.4, 2 ati 10 mg / mL) sinu sẹẹli luminescent (pẹlu ifipamọ apẹẹrẹ bi iṣakoso), ati lẹhinna tẹ 6.25 × 10-4mol / L pyrogallol 0.05 Nikẹhin, 0.94 milimita ti adalu luminol ati ifisi carbonic acid ni a fi kun lati bẹrẹ iṣesi (30 ° C), kikankikan luminescence ni awọn aaye arin ti 2 s, ati apapọ kikankikan luminescence apapọ ni 300 s ni iwọn.Kikan luminescence abẹlẹ jẹ luminescence nigbati a ko ṣafikun pyrogallol.iye.Ni afikun, awọn chemiluminescence wa, fluorescence spectroscopy ati NBS-dichlorofluorescein chemiluminescence eto fun ipinnu ti fluorescence chemiluminescence, ati bẹbẹ lọ, anfani ti o tobi julọ wọn jẹ ifamọ giga.

Kemikali Properties: Yellow crystalline lulú.Ni irọrun tiotuka ninu lye, tiotuka ninu acid dilute, ti o fẹrẹ jẹ insoluble ninu omi, ati insoluble ninu oti.Awọn ojutu aiduro tabi ekikan die-die ṣe afihan fifẹ bulu didan to lagbara nigbati o farahan si ina ultraviolet.Ojuami yo 329-332℃

Nlo: Bi reagent onínọmbà kemikali ati itọkasi.Lo reagent iṣawari itupalẹ Chemiluminescence (gẹgẹbi ipinnu awọn ohun elo irin tabi ẹjẹ)

Nlo: Fun itupalẹ chemiluminescence, gẹgẹbi: awọn cations irin, ẹjẹ ati glucocorticoids

Nlo: Idanwo Luminescence: Emmax440nm (chemiluminescence; 60mmK2S2O8, 100mmK2CO3, pH11.5; lẹhin fifi H2O2) chemiluminescence reagent ati itọkasi, ti a lo nigbagbogbo ni itupalẹ chemiluminescence, gẹgẹbi awọn cations irin, ajesara ẹjẹ, ati bẹbẹ lọ.