3- (1-pyridinio) -1-propanesulfonate Cas: 15471-17-7 99% funfun lulú
| Nọmba katalogi | XD90192 |
| Orukọ ọja | 3- (1-pyridinio) -1-propanesulfonate |
| CAS | 15471-17-7 |
| Ilana molikula | C8H11NO3S |
| Òṣuwọn Molikula | 201.24 |
| Awọn alaye ipamọ | 0 si 8°C |
| Ti irẹpọ Owo idiyele koodu | 29333990 |
Ọja Specification
| Ifarahan | funfun lulú |
| Ayẹwo | 99% |
Amuaradagba phosphorylation ṣe ilana awọn iṣẹ cellular Oniruuru ati pe o ṣe ipa pataki ninu idagbasoke ibẹrẹ ti awọn irugbin.Lati ṣe afikun ati faagun lori awọn iwadii iṣaaju ti phosphorylation amuaradagba ni awọn irugbin Arabidopsis a lo ọna yiyan ti o ṣajọpọ isediwon amuaradagba labẹ awọn ipo ti kii ṣe denaturing pẹlu imudara irin-ion affinity chromatography (IMAC) ti imudara phosphoproteins mule ninu awọn iyọkuro ti Rubisco-depleted, atẹle nipa idanimọ lilo gel electrophoresis onisẹpo meji (2-DE) ati omi kiromatogirafi-tandem mass spectrometry (LC-MS/MS).In-gel trypsin digestion ati itupalẹ awọn aaye gel ti a yan ni idanimọ awọn peptides phosphorylated 144 ati awọn iṣẹku, eyiti 18 phosphopeptides nikan ati awọn phosphosites 8 nikan ni a rii ni PhosPhAt 4.0 ati P3DB Arabidopsis thaliana phosphorylation awọn aaye data data.Die e sii ju idaji awọn phosphoprotein 82 ti a mọ ni o ni ipa ninu iṣelọpọ agbara carbohydrate, photosynthesis/mimi tabi idahun aapọn oxidative mechani sms.Imudara ti awọn phosphoprotein ti ko tọ ṣaaju 2-DE ati LC-MS/MS han lati jẹki wiwa ti threonine phosphorylated ati awọn iṣẹku tyrosine ni akawe pẹlu awọn ọna ti o nlo imudara ipele-peptide, ni iyanju pe awọn isunmọ meji jẹ ibaramu diẹ ni awọn ofin ti aaye agbegbe phosphorylation.Ifiwera awọn abajade fun awọn irugbin ọdọ pẹlu awọn ti a gba ni iṣaaju fun awọn ewe Arabidopsis ti o dagba ṣe idanimọ awọn ọlọjẹ marun ti o yatọ si phosphorylated ninu awọn tisọ wọnyi, ti n ṣe afihan agbara ti ilana yii fun ṣiṣewadii awọn ipa ti phosphorylation amuaradagba lakoko idagbasoke ọgbin.
