Awọn ọja

1.Optically ti nṣiṣe lọwọ D-2-hydroxybutanoate jẹ agbedemeji ile-iṣẹ pataki fun awọn oogun ati poli biodegradable (2-hydroxybutanoate).Ipinnu kinetic ti racemic 2-hydroxybutanoate le jẹ alawọ ewe ati yiyan ti o fẹ fun iṣelọpọ D-2-hydroxybutanoate.Ninu iṣẹ yii, D-2-hydroxybutanoate ni ifọkansi giga (0.197 M) ati apọju enantiomeric giga (99.1%) ni iṣelọpọ nipasẹ L-lactate dehydrogenase olominira NAD (L-iLDH) ti o ni biocatalyst.2-Oxobutanoate, agbedemeji pataki miiran, ni a gbejade ni ifọkansi giga (0.193 M).Lilo ilana paṣipaarọ ion ti o rọrun pẹlu resini paṣipaarọ anion macroporous D301, D-2-hydroxybutanoate ti yapa kuro ninu eto biotransformation pẹlu imularada giga ti 84.7%.

2.Asymmetric kolaginni ti ẹya atubotan amino acid ti a afihan nipa omega-transaminase lati Vibrio fluvialis JS17.L-2-Aminobutyric acid jẹ iṣelọpọ lati 2-oxobutyric acid ati benzylamine pẹlu afikun enantiomeric ti o ga ju 99%.Idahun naa ṣe afihan idinamọ ọja ti o nira nipasẹ benzaldehyde, eyiti o bori nipasẹ lilo eto ifasẹ biphasic lati yọ ọja idilọwọ kuro ni ipele olomi.Ninu ifaseyin biphasic aṣoju (50 mM 2-oxobutyric acid, 70 mM benzylamine ati 2.64 U / ml enzymu ti a sọ di mimọ) nipa lilo hexane bi olutọpa, iyipada ti 2-oxobutyric acid de 96% ni 5 h lakoko ti iyipada 39% nikan ni a gba laisi isediwon ọja.